The 5′-end terminus (1–55543 bp) of the MPXV strain Congo_2003_358 (GenBank accession DQ011154.1) genome was chemically synthesized. The synthesized viral genomic DNA includes four continuous segments. Each viral segment was approximately 15 kb in length (Fig. 3A), with 100 bp of overlap between adjacent segments. 合成的
In this study, although a full-length viral genome would be the ideal reference template for detecting MPXV by qPCR, we only sought to assemble a 55-kb viral fragment, less than one-third of the MPXV genome.
This assembly product is fail-safe by virtually eliminating any risk of recovering into an infectious virus while providing multiple qPCR targets for detecting MPXV or other Orthopoxviruses (Li et al., 2010).
又來？
這看上去還真比較像栽贓……
如果只是要測試 我們會拿手邊方便拿到的材料來進行
病毒研究所不缺病毒
為何要拿一個中國沒有爆發過疫情的病毒 還說這是用合成取得 然後之後又剛好爆發相關疫情.......
按照這論文的內容 沒有使用猴痘病毒的必要性 隨便找一個genome大的痘病毒都可以 我不相信這病毒研究所沒有其他痘病毒 還得用合成方式取得部分序列 (肯定有牛痘病毒)
原來不是有人去幹猴子才爆炸的嗎這個實驗室的工作就是研發生化武器嘛中共死不解封的理由難道是實驗室需要一顆飛彈